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nestin 10c2  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology nestin 10c2
    Nestin 10c2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 959 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nestin+10c2/nestin+Antibody/pm41355579-583-67-71
    Average 96 stars, based on 959 article reviews
    nestin 10c2 - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Immunostaining:

    Article Title: Metformin Restores Mitochondrial Function and Neurogenesis in POLG Patient‐Derived Brain Organoids
    Article Snippet: Images were analyzed using the Leica TCS SP8 STED 3× (Leica Microsystems). .. The following primary antibodies were used for immunostaining: MAP2 (chicken,1:1000; Abcam, ab5392), β‐tubulin III (TUJ1) (mouse, 1:1000; Abcam, ab78078), NeuN (rabbit, 1:500, Cell Signaling Technology, 24 307), BAX (mouse, 1:500; Abcam, ab216494), Cleaved Caspase 3 (mouse, 1:200; Cell Signaling Technology, 9661), POLG (rabbit, 1:500, Abcam, ab128899), POLG 2 (rabbit, 1:500, Abcam, ab227902), GFAP (chicken, 1:200; Abcam, ab4674), VDAC1 (mouse, 1:500; Abcam, ab186321), NDUFB10 (rabbit, 1:1000, Abcam, ab196019), NESTIN (10c2) (mouse, 1:50 Santa Cruz Biotechnology, sc23927), PSD95(mouse, 1:1500, Abcam, ab2723), Synaptophysin (rabbit, 1:500, Abcam, ab32127). .. Alexa Fluor Dyes (Invitrogen) were used at a 1:800 dilution as secondary antibodies.

    Article Title: Metformin Restores Mitochondrial Function and Neurogenesis in POLG Patient-Derived Brain Organoids.
    Article Snippet: Images were analyzed using the Leica TCS SP8 STED 3× (Leica Microsystems). .. The following primary antibodies were used for immunostaining: MAP2 (chicken,1:1000; Abcam, ab5392), β-tubulin III (TUJ1) (mouse, 1:1000; Abcam, ab78078), NeuN (rabbit, 1:500, Cell Signaling Technology, 24 307), BAX (mouse, 1:500; Abcam, ab216494), Cleaved Caspase 3 (mouse, 1:200; Cell Signaling Technology, 9661), POLG (rabbit, 1:500, Abcam, ab128899), POLG 2 (rabbit, 1:500, Abcam, ab227902), GFAP (chicken, 1:200; Abcam, ab4674), VDAC1 (mouse, 1:500; Abcam, ab186321), NDUFB10 (rabbit, 1:1000, Abcam, ab196019), NESTIN (10c2) (mouse, 1:50 Santa Cruz Biotechnology, sc23927), PSD95(mouse, 1:1500, Abcam, ab2723), Synaptophysin (rabbit, 1:500, Abcam, ab32127). .. Alexa Fluor Dyes (Invitrogen) were used at a 1:800 dilution as secondary antibodies.

    other:

    Article Title: Telocytes in human epicardium
    Article Snippet: We determined on serial sections the expression of: CD117 (polyclonal, Dako, 1:250), vimentin (clone V9, Dako, 1:50), CD34 (clone QBend 10, Dako, 1:50), S100 (polyclonal rabbit, Dako, Glostrup Denmark, 1:400), Nestin (clone 10c2, Santa Cruz Biotechnology, Santa Cruz, CA, USA 1:500) and CD57 (clone TB01 Dako, 1:50).

    Article Title: ERG and nestin: useful markers of immature vessels and novel prognostic markers in renal cell carcinoma
    Article Snippet: Nestin , 10c2 , Santa Cruz Biotechnology, Dallas, TX, USA.



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    (A) Schematic illustration of annotated and putative functional domains of NSD1. NID, nuclear receptor interaction domain; PWWP, Pro-Trp-Trp-Pro domain; RBD, RNA-binding domain; PHD, plant homeodomain; AWS, associated with SET domain (also referred to as pre-SET); SET, su(var), enhancer of zeste, trithorax domain. (B) Western blot of NSD1, GAPDH, and H3K36me2 for WT or NSD1/2-dKO HEK293T cells stably rescued with WT or NSD1 mutants. (C) Top: meta-analysis profiling of genome-wide NSD1 and H3K36me2 ChIP-seq signals within −10 kb of TSS to +10 kb of TES. Bottom: representative track images of NSD1-WT or NSD1 ΔPWWP2 in HEK293T NSD1/2-dKO rescued background. TSS, transcription start site; TES, transcription end site. (D) Western blot of NSD1, GAPDH, and H3K36me2 for WT of NSD1/2-dKO HEK293T cells stably rescued with NSD1 or NSD1 PWWP2–4A . (E) Western blot of <t>Nestin,</t> GAPDH, and H3K36me2 for E14-mESC cells undergoing embryoid bodies (EBs) and neural progenitor cell (NPC) differentiation. (F) NPC differentiation of E14-mESCs with indicated genotypes. Top: representative images of (EBs) undergoing NPC differentiation after 3 days of retinoic acid (RA) treatment. Bottom: quantifications of differentiating and non-differentiating EBs. Scale bars, 500 μm.
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    (A) Schematic illustration of annotated and putative functional domains of NSD1. NID, nuclear receptor interaction domain; PWWP, Pro-Trp-Trp-Pro domain; RBD, RNA-binding domain; PHD, plant homeodomain; AWS, associated with SET domain (also referred to as pre-SET); SET, su(var), enhancer of zeste, trithorax domain. (B) Western blot of NSD1, GAPDH, and H3K36me2 for WT or NSD1/2-dKO HEK293T cells stably rescued with WT or NSD1 mutants. (C) Top: meta-analysis profiling of genome-wide NSD1 and H3K36me2 ChIP-seq signals within −10 kb of TSS to +10 kb of TES. Bottom: representative track images of NSD1-WT or NSD1 ΔPWWP2 in HEK293T NSD1/2-dKO rescued background. TSS, transcription start site; TES, transcription end site. (D) Western blot of NSD1, GAPDH, and H3K36me2 for WT of NSD1/2-dKO HEK293T cells stably rescued with NSD1 or NSD1 PWWP2–4A . (E) Western blot of <t>Nestin,</t> GAPDH, and H3K36me2 for E14-mESC cells undergoing embryoid bodies (EBs) and neural progenitor cell (NPC) differentiation. (F) NPC differentiation of E14-mESCs with indicated genotypes. Top: representative images of (EBs) undergoing NPC differentiation after 3 days of retinoic acid (RA) treatment. Bottom: quantifications of differentiating and non-differentiating EBs. Scale bars, 500 μm.
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    Image Search Results


    (A) Schematic illustration of annotated and putative functional domains of NSD1. NID, nuclear receptor interaction domain; PWWP, Pro-Trp-Trp-Pro domain; RBD, RNA-binding domain; PHD, plant homeodomain; AWS, associated with SET domain (also referred to as pre-SET); SET, su(var), enhancer of zeste, trithorax domain. (B) Western blot of NSD1, GAPDH, and H3K36me2 for WT or NSD1/2-dKO HEK293T cells stably rescued with WT or NSD1 mutants. (C) Top: meta-analysis profiling of genome-wide NSD1 and H3K36me2 ChIP-seq signals within −10 kb of TSS to +10 kb of TES. Bottom: representative track images of NSD1-WT or NSD1 ΔPWWP2 in HEK293T NSD1/2-dKO rescued background. TSS, transcription start site; TES, transcription end site. (D) Western blot of NSD1, GAPDH, and H3K36me2 for WT of NSD1/2-dKO HEK293T cells stably rescued with NSD1 or NSD1 PWWP2–4A . (E) Western blot of Nestin, GAPDH, and H3K36me2 for E14-mESC cells undergoing embryoid bodies (EBs) and neural progenitor cell (NPC) differentiation. (F) NPC differentiation of E14-mESCs with indicated genotypes. Top: representative images of (EBs) undergoing NPC differentiation after 3 days of retinoic acid (RA) treatment. Bottom: quantifications of differentiating and non-differentiating EBs. Scale bars, 500 μm.

    Journal: Cell reports

    Article Title: Paraspeckle protein NONO regulates active chromatin by allosterically stimulating NSD1

    doi: 10.1016/j.celrep.2025.116247

    Figure Lengend Snippet: (A) Schematic illustration of annotated and putative functional domains of NSD1. NID, nuclear receptor interaction domain; PWWP, Pro-Trp-Trp-Pro domain; RBD, RNA-binding domain; PHD, plant homeodomain; AWS, associated with SET domain (also referred to as pre-SET); SET, su(var), enhancer of zeste, trithorax domain. (B) Western blot of NSD1, GAPDH, and H3K36me2 for WT or NSD1/2-dKO HEK293T cells stably rescued with WT or NSD1 mutants. (C) Top: meta-analysis profiling of genome-wide NSD1 and H3K36me2 ChIP-seq signals within −10 kb of TSS to +10 kb of TES. Bottom: representative track images of NSD1-WT or NSD1 ΔPWWP2 in HEK293T NSD1/2-dKO rescued background. TSS, transcription start site; TES, transcription end site. (D) Western blot of NSD1, GAPDH, and H3K36me2 for WT of NSD1/2-dKO HEK293T cells stably rescued with NSD1 or NSD1 PWWP2–4A . (E) Western blot of Nestin, GAPDH, and H3K36me2 for E14-mESC cells undergoing embryoid bodies (EBs) and neural progenitor cell (NPC) differentiation. (F) NPC differentiation of E14-mESCs with indicated genotypes. Top: representative images of (EBs) undergoing NPC differentiation after 3 days of retinoic acid (RA) treatment. Bottom: quantifications of differentiating and non-differentiating EBs. Scale bars, 500 μm.

    Article Snippet: Nestin mouse monoclonal 10C2 , Cell Signaling Technology , Cat# 33475.

    Techniques: Functional Assay, RNA Binding Assay, Western Blot, Stable Transfection, Genome Wide, ChIP-sequencing